2. The film was shot on digital video while Darren was going to graduate school at The American Film Institute in 1992-1993. It was his thesis project and when it was screened he got a standing ovation in the theater.
3. The credits for Protozoa give a special thanks to:
4. Darren's production company Protozoa Pictures is named after this student film.
5. Michael Bonitatis (Dave) sliced his finger while working on the opening scene, and wears a bandage for the rest of the film.
6. In an interview, Darren stated that he enjoyed the film Charlie's Angels. That film featured Lucy Liu, who also appeared in Protozoa